Application

Use biotin-16-UTP to add a nonradioactive label to RNA during in vitro transcription, so the RNA can easily and safely be detected (e.g., with a fluorescein-conjugated or enzyme-conjugatedstreptavidin). Biotin-16-UTP can replace UTP as a substrate for the following enzymes:

• SP6 RNA polymerase
• T3 RNA polymerase
• T7 RNA polymerase

Product Description

Brief description: Biotin is bound to uridine triphosphate via an amide linkage.

Formula: C₃₂H₄₈N₇O₁₉P₃SLi₄

Molecular weight: M_r =987.5 (biotin-16-UTP-Li₄)

Background Information

Biotin-labeled RNA can be used as a hybridization probe for:

• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization

Contents

Biotin-16-UTP, tetralithium salt; 10 mM solution

Quality

Typical analysis: at least 85% Biotin-16-UTP (HPLC, area %).